ASSESSMENT OF BIOAEROSOL CONTAMINATION IN AN URBAN WASTEWATER TREATMENT PLANT IN TEHRAN, IRAN

  • Majid Kermani Research Center for Environmental Health Technology, Iran University of Medical Sciences, Tehran, Iran AND Associate Professor of Environmental Health Engineering Department, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
  • Anahita Dehghani Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran AND MSc of Environmental Health Engineering, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran
  • Mahdi Farzadkia Research Center for Environmental Health Technology, Iran University of Medical Sciences, Tehran, Iran AND Professor of Environmental Health Engineering Department, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
  • Farshad Bahrami Asl PhD student of Environmental Health Engineering Department, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
  • Dariush Zeinalzadeh MSc of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Air contamination, bioaerosols, wastewater treatment plant, Tehran

Abstract

Introduction: Recently contact with bioaerosols has been presented as an important problem which endangers human being’s health. In this study bioaerosol concentration was measured in wastewater treatment (WWTP) units in west of Tehran.

Materials and methods:
Passive sampling was carried out around three process and operational units, in 100 m from last unit of wastewater treatment plant and in administrative building. In total 440 samples were collected. The transport culture medium used for bacterial samples was the tryptic soy agar, and for fungal samples, it was dextrose agar. Sampling was carried out according to the sampling calendar of Environmental Protection Agency (EPA) in 2013 for 1 h every 6 days and in the plates of 9 cm.

Results:
The results showed that aerobic digester with an average of 3303 CFU/Plate had the greatest effect on emission of bacterial bioaerosols. In addition grit chamber with an average of 586.3 CFU/Plate had a highest impact on fungal emission. Among the bacteria, Bacillus spp. Staphylococcus spp. and Micrococcus spp. were dominant. The highest emission of bacterial aerosol is in July and the lowest is in March. Furthermore, fungi such as Cladosporium spp. Penicillium spp. and Alternaria spp. were the dominant types in the wastewater treatment plant. The highest emission of fungal aerosols is in March and the lowest emission is in July.

Conclusions:
According to the results, operational and processing units of WWTP influence pollution load and dispersion of bioaerosols. Therefore, appropriate environmental health management in WWTP could be one of the important factors reducing dispersion of bioaerosols.

References

Fannin KF, Vana SC, Jakubowski W. Effect of an activated sludge wastewater treatment plant on ambient air densities of aerosols containing bacteria and viruses. Applied and environmental microbiology. 1985;49(5):1191-6.

Dehghani A, Kermani M, Farzadkia M, Naddafi K, Alimohammadi M. A comparative study for potential of microbial pollution in the ambient air of Milad hosbital, blood transfusion organization and Tehran’s Shahrake Gharb wastewater treatment plant. Journal of Urmia Nursing and Midwifery Faculty. 2014;12(3):183-92.

Li L, Gao M, Liu J. Distribution characterization of microbial aerosols emitted from a wastewater treatment plant using the Orbal oxidation ditch process. Process Biochemistry. 2011;46(4):910-5.

Kermani M, Dehghani A, Farzadkia M, Nadafi K, Bahrami Asl F, Zeinalzadeh D. Investigation of airborne bactria and fungi in Tehran’s Shahrake Ghods WWTP and its association with environmental parameters. Journal of Health. 2015;6(1):57-68.

Sánchez-Monedero M, Aguilar M, Fenoll R, Roig A. Effect of the aeration system on the levels of airborne microorganisms generated at wastewater treatment plants. Water Research. 2008;42(14):3739-44.

Carducci A, Tozzi E, Rubulotta E, Casini B, Cantiani L, Rovini E, et al. Assessing airborne biological hazard from urban wastewater treatment. Water Research. 2000;34(4):1173-8.

Kruczalak K, Olanczuk-Neyman K. Microorganisms in the air over wastewater treatment plants. Polish Journal of Environmental Studies. 2004;13(5):537-42.

Lee JH, Jo W-K. Characteristics of indoor and outdoor bioaerosols at Korean high-rise apartment buildings. Environmental Research. 2006;101(1):11-7.

Orsini M, Laurenti P, Boninti F, Arzani D, Ianni A, Romano- Spica V. A molecular typing approach for evaluating bioaerosol exposure in wastewater treatment plant workers. Water Research. 2002;36(5):1375-8.

Heinonen-Tanski H, Reponen T, Koivunen J. Airborne enteric coliphages and bacteria in sewage treatment plants. Water Research. 2009;43(9):2558-66.

Breza-Boruta B, Paluszak Z. Influence of water treatment plant on microbiological composition of air bioaerosol. Polish Journal of Environmental Studies. 2007;16(5):663.

Wlazło A, Pastuszka J, Łudzeń-Izbińska B. Assessment of workers’ exposure to airborne bacteria at a small wastewater treatment plant. Medycyna Pracy. 2001;53(2):109-14.

Sawyer B, Elenbogen G, Rao K, O’Brien P, Zenz DR, Lue-Hing C. Bacterial aerosol emission rates from municipal wastewater aeration tanks. Applied and environmental microbiology. 1993;59(10):3183-6.

Jensen PA, Schafer MP. Sampling and characterization of bioaerosols. NIOSH manual of analytical methods. 1998;1(15):82-112.

Bauer H, Fuerhacker M, Zibuschka F, Schmid H, Puxbaum H. Bacteria and fungi in aerosols generated by two different types of wastewater treatment plants. Water Research. 2002;36(16):3965-70.

Kim K-Y, Kim H-T, Kim D, Nakajima J, Higuchi T. Distribution characteristics of airborne bacteria and fungi in the feedstuff manufacturing factories. Journal of Hazardous Materials. 2009;169(1):1054-60.

Karra S, Katsivela E. Microorganisms in bioaerosol emissions from wastewater treatment plants during summer at a Mediterranean site. Water Research. 2007;41(6):1355-65.

Filipkowska Z, Janczukowicz W, Krzemieniewski M, Pesta J. Microbiological air pollution in the surroundings of an Ecoblock wastewater treatment plant. Biuletyn Naukowy Uniwersytet Warminsko-Mazurski wOlsztynie (Poland). 2002.

Michałkiewicz M, Pruss A, Dymaczewski Z, Jeż-Walkowiak J, Kwaśna S. Microbiological air monitoring around municipal wastewater treatment plants. Polish Journal of Environmental Studies. 2011;20(5):1243-50.

Małecka-Adamowicz M, Donderski W, Dokładna W. Microflora of air in the sewage treatment plant of kapuściska in Bydgoszcz. Polish J of Environ. 2007;16(1):101-7.

Tang JW. The effect of environmental parameters on the survival of airborne infectious agents. Journal of the Royal Society Interface. 2009;6(Suppl 6):S737-S46.

Filipkowska Z, Janczukowicz W, Krzemieniewski M, Pesta J. Microbiological air pollution in the surroundings of the wastewater treatment plant with activatedsludge tanks aerated by horizontal rotors. Polish Journal of Environmental Studies. 2000;9(4):273-80.

Bünger J, Schappler-Scheele B, Hilgers R, Hallier E. A 5-year follow-up study on respiratory disorders and lung function in workers exposed to organic dust from composting plants. International archives of occupational and environmental health. 2007;80(4):306-12.

Laitinen S, Kangas J, Kotimaa M, Liesivuori J, Martikainen PJ, Nevalainen A, et al. Workers’ exposure to airborne bacteria and endotoxins at industrial wastewater treatment plants. American Industrial Hygiene Association. 1994;55(11):1055-60.

Pascual L, Pérez-Luz S, Yanez MA, Santamaria A, Gibert K, Salgot M, et al. Bioaerosol emission from wastewater treatment plants. Aerobiologia. 2003;19(3-4):261-70.

Korzeniewska E, Filipkowska Z, Gotkowska-Płachta A, Janczukowicz W, Dixon B, Czułowska M. Determination of emitted airborne microorganisms from a BIO-PAK wastewater treatment plant. Water Research. 2009;43(11):2841-51.

Krzysztofik B. Air microbiology. Warsaw University of Technology Publishing House, Warszawa. 1992.

Oppliger A, Hilfiker S, Duc TV. Influence of seasons and sampling strategy on assessment of bioaerosols in sewage treatment plants in Switzerland. Annals of Occupational Hygiene. 2005;49(5):393-400.

Rosas I, Calderón C, Martínez L, Ulloa M, Lacey J. Indoor and outdoor airborne fungal propagule concentrations in Mexico City. Aerobiologia. 1997;13(1):23-30.

Fernando NL, Fedorak PM. Changes at an activated sludge sewage treatment plant alter the numbers of airborne aerobic microorganisms. Water Research. 2005;39(19):4597-608.

Schlesinger P, Mamane Y, Grishkan I. Transport of microorganisms to Israel during Saharan dust events. Aerobiologia. 2006;22(4):259-73.

Filipkowska Z, Janczukowicz W, Krzemieniewski M, Pesta J. Municipal wastewater treatment plant with activated sludge tanks aerated by CELPOX devices as a source of microbiological pollution of the atmosphere. Polish Journal of Environmental Studies. 2002;11(6):639-48.

Published
2016-09-04
How to Cite
1.
Kermani M, Dehghani A, Farzadkia M, Bahrami Asl F, Zeinalzadeh D. ASSESSMENT OF BIOAEROSOL CONTAMINATION IN AN URBAN WASTEWATER TREATMENT PLANT IN TEHRAN, IRAN. japh. 1(3):161-170.
Section
Original Research