Tehran University of Medical Sciences Journal of Air Pollution and Health 2476-3071 1 4 2017 03 01 259 268 EN Kazem Naddafi Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran AND Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Zahra Atafar Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran AND Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Maryam Faraji Environmental Health Engineering Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Maryam Ghanbarian Environmental Health Engineering Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Soheila Rezaei Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ghanbari Ghozikali Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, IranAND Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Hassanvand Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran AND Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Zahra Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran Alireza Mesdaghinia Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran AND Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Masoud Yunesian Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Kamyar Yaghmaeian Environmental Health Engineering Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ramin Nabizadeh Nodehi Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Nicknam Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranAND Department of Research Methodology and Data Analysis, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran Mirzaman Zamanzadeh Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mansour Shamsipour Department of Research Methodology and Data Analysis, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran Khalil Ansarin Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2017 01 14 2017 02 04 Introduction: Air pollution is a serious health concern worldwide, accounting for high premature morbidity and mortality annually. In this article, we explain the framework of a series of interrelated researches for studying particulate matter (PM10, PM <10 μm) health impacts from dust storm and non-dust storm conditions in Tehran, Iran. Materials and methods: PM10 samples will be collected daily from two representative stations simultaneously during normal and dusty days by high volume samplers. PM chemical properties including ions, polycyclic aromatic hydrocarbons, metal (loid)s and oxidative potential will be analyzed. For in vitro biological toxicity, cytokine release from peripheral blood mononuclear cells and A549 cell line will be assayed. Also, DNA damage on A549 cell line will be examined using comet assay. In addition, DNA methylation of PBMCs and hemolysis of red blood cells (RBCs) will be accomplished. In a parallel panel study, after completing demographic and the International Study of Asthma and Allergies in Childhood (ISSAC) questionnaires, two groups of students will be selected. The exhaled breath condensate will be conducted to measure TNF-α, 8-IP, IL-6. Conclusions: This study aimed to evaluate the components, oxidative potential and biological effects induced by PM. Our research will be provided information on the toxicity potential of PM related to dust storm and traffic to help policy makers to establish strategies and prioritize resource allocation in comprehensive plan for PM control. https://japh.tums.ac.ir/index.php/japh/article/view/74 https://japh.tums.ac.ir/index.php/japh/article/download/74/39