Tehran University of Medical Sciences Journal of Air Pollution and Health 2476-3071 10 4 2025 12 28 543 558 Abbas Jafari Department of Occupational Health, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran AND Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran Soma Karimi Department of Occupational Health, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran Zeinab Esmaeilzadeh Department of Nutrition, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran Mohammad Hajaghazadeh Social Determinants of Health Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran 2025 11 13 2025 12 09 Introduction: Perchloroethylene (PCE) is widely used in dry cleaning and has been linked to hepatorenal toxicity. We aimed to assess the relationship between occupational PCE exposure, oxidative stress, and biomarkers of liver and kidney function. Materials and methods: We conducted a cross-sectional study of 30 male Iranian dry-cleaning workers and 30 frequency-matched controls. Personal full-shift air samples were collected for PCE. Serum biomarkers of oxidative stress ; Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (CAT); and organ function; Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline Phosphatase (ALP), bilirubin, creatinine, urea; were measured. Results: Exposed workers had a mean Time-Weighted Average (TWA) of 29 ppm, exceeding the 25-ppm Occupational Exposure Limit (OEL). Compared with controls, Malondialdehyde (MDA), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and creatinine were higher (p<0.05), while SOD and CAT were lower (p<0.05); ALP and bilirubin did not differ. Within the exposed group, longer employment was associated with worsening oxidative and hepatorenal markers. Multivariate regression analysis confirmed that PCE exposure remained a significant independent predictor of oxidative stress (MDA, SOD, CAT) even after adjusting for age and smoking. Conclusion: Findings indicate oxidative stress accompanies subclinical liver injury and early renal impact at prevailing occupational exposures. Reducing PCE through engineering controls and safer technologies should be prioritized https://japh.tums.ac.ir/index.php/japh/article/view/1151 https://japh.tums.ac.ir/index.php/japh/article/download/1151/456